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geneart strings dna fragments  (Thermo Fisher)


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    Structured Review

    Thermo Fisher geneart strings dna fragments
    Geneart Strings Dna Fragments, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/geneart+strings+dna+fragments/DNA/pmc12758139-173-17-17
    Average 99 stars, based on 1 article reviews
    geneart strings dna fragments - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Synthesized:

    Article Title: Stabilized Ion Selectivity Corrects Activation Drift in Kalium Channelrhodopsins
    Article Snippet: .. [ ] The WiChR DNA was de novo synthesized (GeneArt Strings DNA Fragments, Thermo Fisher Scientific) with additional sequences for BamHI and XhoI (restriction enzymes) at the N‐terminal and C‐terminal respectively. ..

    Article Title: Optogenetic induction of subcellular Ca 2+ events in megakaryocytes and platelets using a highly Ca 2+ -conductive channelrhodopsin.
    Article Snippet: .. The CapChR2 was generated by quick change point mutations by Chong Zhang of the Nagel/ Gao group with the CoChR L112C plasmid synthesized by GeneArt Strings DNA Fragments (Life Technologies, Thermo Fisher Scientific), according to the published amino acid sequences13. .. Plasmids for Xenopus oocyte expression were linearized by NheI digestion. cRNAs were generated by in vitro transcription with the AmpliCap-MaxT7 High Yield Message Maker Kit (Epicentre Biotechnologies), using the linearized plasmid DNA as templates.

    Article Title: Synchronization of the segmentation clock using synthetic cell–cell signaling
    Article Snippet: Nucleotide fragments of EGFP and hPDGFRB(512–561) were PCR-amplified from pHR-EGFPligand (a generous gift from Wendell A. Lim; Addgene 79129). .. Nucleotide fragments for PCR amplification of LaM4, LaM8, HaloTag, mScarlet-I, SNAP, and emiRFP670 were synthesized by the “GeneArt strings DNA fragments” service (Thermo Fisher Scientific) with humanized codon optimization. ..

    Article Title: Stabilized Ion Selectivity Corrects Activation Drift in Kalium Channelrhodopsins.
    Article Snippet: .. [29] TheWiChR DNA was de novo synthesized (GeneArt Strings DNA Fragments, Thermo Fisher Scientific) with additional sequences for BamHI and XhoI (restriction enzymes) at the N-terminal and C-terminal respectively. ..

    Article Title: Synchronization of the segmentation clock using synthetic cell-cell signaling.
    Article Snippet: Nucleotide fragments of EGFP and hPDGFRB(512–561) were PCR-amplified frompHR-EGFPligand (a generous gift fromWendell A. Lim; Addgene 79129). .. Nucleotide fragments for PCR amplification of LaM4, LaM8, HaloTag, mScarlet-I, SNAP, and emiRFP670 were synthesized by the “GeneArt strings DNA fragments” service (Thermo Fisher Scientific) with humanized codon optimization. ..

    Generated:

    Article Title: Optogenetic induction of subcellular Ca 2+ events in megakaryocytes and platelets using a highly Ca 2+ -conductive channelrhodopsin.
    Article Snippet: .. The CapChR2 was generated by quick change point mutations by Chong Zhang of the Nagel/ Gao group with the CoChR L112C plasmid synthesized by GeneArt Strings DNA Fragments (Life Technologies, Thermo Fisher Scientific), according to the published amino acid sequences13. .. Plasmids for Xenopus oocyte expression were linearized by NheI digestion. cRNAs were generated by in vitro transcription with the AmpliCap-MaxT7 High Yield Message Maker Kit (Epicentre Biotechnologies), using the linearized plasmid DNA as templates.

    Plasmid Preparation:

    Article Title: Optogenetic induction of subcellular Ca 2+ events in megakaryocytes and platelets using a highly Ca 2+ -conductive channelrhodopsin.
    Article Snippet: .. The CapChR2 was generated by quick change point mutations by Chong Zhang of the Nagel/ Gao group with the CoChR L112C plasmid synthesized by GeneArt Strings DNA Fragments (Life Technologies, Thermo Fisher Scientific), according to the published amino acid sequences13. .. Plasmids for Xenopus oocyte expression were linearized by NheI digestion. cRNAs were generated by in vitro transcription with the AmpliCap-MaxT7 High Yield Message Maker Kit (Epicentre Biotechnologies), using the linearized plasmid DNA as templates.

    cDNA Library Assay:

    Article Title: Triple-helical polypeptides lacking binding affinity for the Fc domain of immunoglobulin and uses thereof
    Article Snippet: .. The corresponding cDNA library for PA02 and PA03 was provided by ThermoFisher Scientific as GeneArt Strings DNA Fragments. ..

    Polymerase Chain Reaction:

    Article Title: Synchronization of the segmentation clock using synthetic cell–cell signaling
    Article Snippet: Nucleotide fragments of EGFP and hPDGFRB(512–561) were PCR-amplified from pHR-EGFPligand (a generous gift from Wendell A. Lim; Addgene 79129). .. Nucleotide fragments for PCR amplification of LaM4, LaM8, HaloTag, mScarlet-I, SNAP, and emiRFP670 were synthesized by the “GeneArt strings DNA fragments” service (Thermo Fisher Scientific) with humanized codon optimization. ..

    Article Title: Synchronization of the segmentation clock using synthetic cell-cell signaling.
    Article Snippet: Nucleotide fragments of EGFP and hPDGFRB(512–561) were PCR-amplified frompHR-EGFPligand (a generous gift fromWendell A. Lim; Addgene 79129). .. Nucleotide fragments for PCR amplification of LaM4, LaM8, HaloTag, mScarlet-I, SNAP, and emiRFP670 were synthesized by the “GeneArt strings DNA fragments” service (Thermo Fisher Scientific) with humanized codon optimization. ..

    Amplification:

    Article Title: Synchronization of the segmentation clock using synthetic cell–cell signaling
    Article Snippet: Nucleotide fragments of EGFP and hPDGFRB(512–561) were PCR-amplified from pHR-EGFPligand (a generous gift from Wendell A. Lim; Addgene 79129). .. Nucleotide fragments for PCR amplification of LaM4, LaM8, HaloTag, mScarlet-I, SNAP, and emiRFP670 were synthesized by the “GeneArt strings DNA fragments” service (Thermo Fisher Scientific) with humanized codon optimization. ..

    Article Title: Synchronization of the segmentation clock using synthetic cell-cell signaling.
    Article Snippet: Nucleotide fragments of EGFP and hPDGFRB(512–561) were PCR-amplified frompHR-EGFPligand (a generous gift fromWendell A. Lim; Addgene 79129). .. Nucleotide fragments for PCR amplification of LaM4, LaM8, HaloTag, mScarlet-I, SNAP, and emiRFP670 were synthesized by the “GeneArt strings DNA fragments” service (Thermo Fisher Scientific) with humanized codon optimization. ..

    Sequencing:

    Article Title: Calcium-dependent activation of TREK-1 and TREK-2 background potassium channels by calcineurin
    Article Snippet: In vitro site-directed mutagenesis was performed according to the manufacturer’s instructions of the QuikChangeTM site-directed mutagenesis kit (Stratagene, La Jolla, CA), but by applying non-overlapping primer pairs with sufficient 3′ overhangs as required by the Phusion polymerase . .. Extended sequence modifications were assembled by the application of partially overlapping oligonucleotides or ordered as Invitrogen GeneArt Strings DNA Fragments (Thermo Fisher). ..

    Article Title: Calcium-dependent activation of TREK-1 and TREK-2 background potassium channels by calcineurin.
    Article Snippet: In vitro site-directed mutagenesis was performed according to the manufacturer's instructions of the QuikChangeTM site-directed mutagenesis kit (Stratagene, La Jolla, CA), but by AR TIC LE IN PR ES S 26 applying non-overlapping primer pairs with sufficient 3’ overhangs as required by the Phusion polymerase68. .. Extended sequence modifications were assembled by the application of partially overlapping oligonucleotides or ordered as Invitrogen GeneArt Strings DNA Fragments (Thermo Fisher). ..



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